The measurement of thiamine in urine.
نویسندگان
چکیده
The most widely used procedures for the measurement of thiamine in urine are based on the oxidation of the vitamin to thiochrome, which is then extracted into butyl alcohol and measured fluorometrically (1). It has been found possible to omit the butyl alcohol extraction without prejudice to the assay. By utilizing benzenesulfonyl chloride to obtain blank values, as proposed by Urban and Goldman (2), as well as by incorporating certain other minor changes, a procedure has been developed which appears to combine convenience and simplicity with accuracy.
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ورودعنوان ژورنال:
- The Journal of biological chemistry
دوره 195 1 شماره
صفحات -
تاریخ انتشار 1952